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Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
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Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
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Raytest GmbH aida software version 4.50.010
Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
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Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
Ic50 Value, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc non-linear regression curve fit
Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
Non Linear Regression Curve Fit, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Greiner Bio c 8216 leucosep tubes
Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
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GraphPad Software Inc graphpad prism software
Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
Graphpad Prism Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VILBER GmbH fusion solo imaging system
Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to <t>TMZ</t> (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination <t>with</t> <t>DMSO</t> or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)
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Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to TMZ (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination with DMSO or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: HSP90-CDC37 functions as a chaperone for the oncogenic FGFR3-TACC3 fusion.

doi: 10.1016/j.ymthe.2022.02.009

Figure Lengend Snippet: Figure 6. Hsp90 inhibitor chemosensitizes F3-T3 glioma cells to TMZ (A) Combination drug matrix for estimates of interactions using CalcuSyn software. LN-229 cells stably expressing F3-T3 were treated (+/) onalespib and TMZ as shown and relative CCK8 viability was measured after 72 h. Percentages are the means from 3 independent experiments and indicate cell death relative to the untreated controls. (B) U-251 MG cells or LN-229 cells stably expressing F3-T3 treated with onalespib or 17-AAG in combination with DMSO or TMZ and assayed for colony formation after 8 days. Mean colonies (treatment/DMSO ±SD (n = 3); ***p < 0.001 each drug alone to each combination by 1-way ANOVA with Tukey’s post-test. (C) U-251 MG cells (legend continued on next page)

Article Snippet: TMZ (5 mg/mL in 20% DMSO) was administered by oral gavage and onalespib (6 mg/mL in 15% SelleckChem Captisol-S4592 in water) by tail vein injection given on the days and in the concentrations indicated in the Figures 7A and S6B) schematic overview.

Techniques: Software, Stable Transfection, Expressing